| Titre : | Preliminary Phytochemical Screening and Evaluation of some biological activities of Bituminaria bituminosa L |
| Auteurs : | MOKHTARI souad, Auteur ; BEN GHERBAL khaoula, Auteur ; Habiba Laraoui, Directeur de thèse |
| Type de document : | Mémoire magistere |
| Editeur : | Biskra [Algérie] : Faculté des Sciences Exactes et des Sciences de la Nature et de la Vie, Université Mohamed Khider, 2022 |
| Format : | 1 vol. (65 p.) / ill.couv.ill.en coul / 30cm |
| Langues: | Anglais |
| Mots-clés: | Bituminaria bituminosa, phenolic, flavonoid content, antimicrobial, antioxidant activity . |
| Résumé : |
This study is devoted to the estimation of total phenolic content, flavonoid content, and the evaluation of in vitro antioxidant and antibacterial activities of crude extract (ethyl acetate EtOAc) obtained from the species Bituminaria bituminosa L. The phytochemical analysis of this plant by using preliminary tests and TLC technic, revealed the presence of many secondary metabolites including: reducing sugars, steroids, flavonoids, tannins, alkaloids, polyphenols and essential oil. In further, the total phenolic and flavonoid contents were determined spectrophotometrically by the Folin–Ciocalteu and trichloroalluminium technics. Indeed, the ethyl acetate extract recorded a good content of polyphenols (133 ± 0.014 GAE/mg of plant extract) and flavonoids (92.20 ± 0.039 μg QE/mg of plant extract). Additionally, the antioxidant activity of crude extract (EtOAc) was evaluated by using DPPH• assay, where the results showed that the tested extract has the ability to scavenge the free radical DPPH with IC50 value: 0.19 ± 0.011 mg/mL. Antibacterial activity was performed according to disc diffusion method. Unfortunately, the crude extract (EtOAc) has no antibacterial effect against all the tested strains compared to Chloramphenicol, Cefotaxime and Tobramycin as positive controls. |
| Sommaire : |
Chapter I : Literature Review I.1. Fabaceae family …………………………………………………………………………...4 I.1.1. Systimatic position ……………………………………………………………………..4 I.1.2. Characteristics features of fabaceae ………………………………………5 I.1.3. Geographical distribution of Fabaceae species…………………………………….6 I.2. Genus Bituminaria « Psoralea » …………………………………………………….....6 I.2.1. Distribution of Bituminaria genus ……………………………………………..……...6 I.2.2. Botanical description of Bituminaria species ………………………………. 7 I.2.3. Traditional uses and pharmacological activities of the genus Bituminaria ………...8 I.2.4. Phytochemistry studies of the genus Bituminaria ………………………………………...9 I.3. Bituminaria bituminosa species …………………………………………………….13 I 3.1. Distribution of Bituminaria bituminosa …………………………………………..13 I 3.2. Botanical description of Bituminaria bituminosa .…………………………………14 I.3.3. Traditional uses of Bituminaria bituminosa ………………………………………...15 I.3.4. Biological effects of Bituminaria bituminosa ………………………………………….16 Chapter I : Literature Review I.3.5. Chemical composition and pharmacological significance of B. bituminosa ………...16 I.4. Biological activities of Medicinal Plants …………………………………………… ...19 I.4.1. Antibacterial activity … ……………………………………………. ……………… 20 I.4.1.1. Bacteria definition ………………………………………………………………… ..20 I.4.1.2. Morphology and classification ……………………………………………………. ..20 I.4.1.3. Methods for in vitro evaluating antimicrobial activity ……………………….........22 I.4.2. Antioxidant activity …………………………………………………………………... 24 I.4.2.1 Methods for in vitro evaluating antioxidant activity ……………………………...25 References ……………..….…………………………………………………………………28 II.1. Plant material ……………………………………………………………………….…31 II.2. Phytochemical screening……………………………………………………………….31 II.3. Extraction techniques …………………………………………………………………...37 II.3.1. Maceration ……………………………………………………………………………37 II.3.2. Filtration and evaporation…………………………………………………………....37 II.3.3. Liquide–liquide extraction (LLE) ……………………………………………………38 II.4. Thin layer chromatography analysis (TLC) …………………………………………….38 II.5. Determination of total phenolic content in EtOAc extract ……………………………..40 II.6. Determination of total flavonoid content in EtOAc extract ...………………………...41 II.7. Biological Activities ……………………………………………………………………42 II.7.1. Antioxidant activity of EtOAc extract …………………………………………….43 II.7.2. Antibacterial activity of EtOAc extract ………………………………………………44 References …….…….………………...……………………………………………………..46 Chapter II : Materials and Methods III.1. Phytochemical screening ………………………………47 III.2. Chromatographic (TLC) analysis of the different obtained extracts…………………..49 III.3. Total phenolic content (TPP) ………………………………………………………….51 III.4. Total flavonoid content (TFV) …………………………………………………..……51 III.5. Antioxidant activity ………………………………………………………………...…52 III.5. Antibacterial activity …………………………………………………………………..53 References …………………..…...…………..………………………………………………55 CONCLUSION …………….……………………………………………………………….56 |
| Type de document : | Mémoire master |
Disponibilité (1)
| Cote | Support | Localisation | Statut |
|---|---|---|---|
| MCH/543 | Mémoire master | bibliothèque sciences exactes | Empruntable |
